Recombinant Human ERBB2 Protein, ECD, Tag Free, Biotinylated

Specification :
| Description : This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. | Source : CHO | Species : Human | Tag : Tag Free | Protein length : ECD | Conjugation/Label : Biotin | Form : Lyophilised | Endotoxin : Endotoxin level was found to be less than 0.1 ng/μg (1 EU/μg). | Applications : ELISA, WB | Notes : FOR RESEARCH USE ONLY (RUO). | Storage : The lyophilized protein is stable for at least 1 year from date of receipt at -20 centigrade. Upon reconstitution, this cytokine can be stored in working aliquots at 2 to 8 centigrade for one month, or at -20 centigrade for six months, with a carrier protein without detectable loss | Storage Buffer : Lyophilised from 0.2 μm filtered PBS pH 7.4. | Reconstitution : Upon receipt a quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.