Recombinant Human ERBB2 Protein, GST-tagged

Specification :
| Description : This gene encodes a member of the epidermal growth factor (EGF) receptor family of receptor tyrosine kinases. This protein has no ligand binding domain of its own and therefore cannot bind growth factors. However, it does bind tightly to other ligand-bound EGF receptor family members to form a heterodimer, stabilizing ligand binding and enhancing kinase-mediated activation of downstream signalling pathways, such as those involving mitogen-activated protein kinase and phosphatidylinositol-3 kinase. Allelic variations at amino acid positions 654 and 655 of isoform a (positions 624 and 625 of isoform b) have been reported, with the most common allele, Ile654/Ile655, shown here. Amplification and/or overexpression of this gene has been reported in numerous cancers, including breast and ovarian tumors. Alternative splicing results in several additional transcript variants, some encoding different isoforms and others that have not been fully characterized. [provided by RefSeq, Jul 2008] | Source : Wheat Germ | Species : Human | Tag : GST | Molecular Mass : 164.45 kDa | AA Sequence : MELAALCRWGLLLALLPPGAASTQV CTGTDMKLRLPASPETHLDMLRHLY QGCQVVQGNLELTYLPTNASLSFLQ DIQEVQGYVLIAHNQVRQVPLQRLR IVRGTQLFEDNYALAVLDNGDPLNN TTPVTGASPGGLRELQLRSLTEILK GGVLIQRNPQLCYQDTILWKDIFHK NNQLALTLIDTNRSRACHPCSPMCK GSRCWGESSEDCQSLTRTVCAGGCA RCKGPLPTDCCHEQCAAGCTGPKHS DCLACLHFNHSGICELHCPALVTYN TDTFESMPNPEGRYTFGASCVTACP YNYLSTDVGSCTLVCPLHNQEVTAE DGTQRCEKCSKPCARVCYGLGMEHL REVRAVTSANIQEFAGCKKIFGSLA FLPESFDGDPASNTAPLQPEQLQVF ETLEEITGYLYISAWPDSLPDLSVF QNLQVIRGRILHNGAYSLTLQGLGI SWLGLRSLRELGSGLALIHHNTHLC FVHTVPWDQLFRNPHQALLHTANRP EDECVGEGLACHQLCARGHCWGPGP TQCVNCSQFLRGQECVEECRVLQGL PREYVNARHCLPCHPECQPQNGSVT CFGPEADQCVACAHYKDPPFCVARC PSGVKPDLSYMPIWKFPDEEGACQP CPINCTHSCVDLDDKGCPAEQRASP LTSIISAVVGILLVVVLGVVFGILI KRRQQKIRKYTMRRLLQETELVEPL TPSGAMPNQAQMRILKETELRKVKV LGSGAFGTVYKGIWIPDGENVKIPV AIKVLRENTSPKANKEILDEAYVMA GVGSPYVSRLLGICLTSTVQLVTQL MPYGCLLDHVRENRGRLGSQDLLNW CMQIAKGMSYLEDVRLVHRDLAARN VLVKSPNHVKITDFGLARLLDIDET EYHADGGKVPIKWMALESILRRRFT HQSDVWSYGVTVWELMTFGAKPYDG IPAREIPDLLEKGERLPQPPICTID VYMIMVKCWMIDSECRPRFRELVSE FSRMARDPQRFVVIQNEDLGPASPL DSTFYRSLLEDDDMGDLVDAEEYLV PQQGFFCPDPAPGAGGMVHHRHRSS STRSGGGDLTLGLEPSEEEAPRSPL APSEGAGSDVFDGDLGMGAAKGLQS LPTHDPSPLQRYSEDPTVPLPSETD GYVAPLTCSPQPEYVNQPDVRPQPP SPREGPLPAARPAGATLERPKTLSP GKNGVVKDVFAFGGAVENPEYLTPQ GGAAPQPHPPPAFSPAFDNLYYWDQ DPPERGAPPSTFKGTPTAENPEYLG LDVPV | Applications : Enzyme-linked Immunoabsorbent AssayWestern Blot (Recombinant protein)Antibody ProductionProtein Array | Notes : Best use within three months from the date of receipt of this protein. | Storage : Store at -80 centigrade. Aliquot to avoid repeated freezing and thawing. | Storage Buffer : 50 mM Tris-HCI, 10 mM reduced Glutathione, pH=8.0 in the elution buffer.