Recombinant Human Fas Ligand soluble, FLAG-tagged

Specification :
| Source : HEK 293 cells | Species : Human | Tag : FLAG | Form : Provided as a lyophilized powder. Contains PBS. | Molecular Mass : ~32 kDa (non-glycosylated), ~35 kDa (glycosylated) (SDS-PAGE) | Endotoxin : Endotoxin content is | Purity : ≥95% purity as determined by SDS-PAGE. | Characteristic : rhsFasL binds to human, mouse and rat Fas. | Applications : Induction of Apoptosis: Recombinant FasL protein induces apoptosis in Fas-sensitive cells (ED50: 50 ng/mL in A20 cells). Optimal concentration varies with cell type and should be determined by testing serial dilutions on cells. Alone, rhsFasL kills Fas-sensitive cells at a concentration of >10 ng/mL. Use of a secondary enhancing cross-linking antibody reacting with rhsFasL increases the activity of rhsFasL approximately 50-fold. | Usage : For in vitro research use only. Not for use in diagnostics or in humans. | Notes : Results using sFasL may differ from those obtained with agonistic antibodies. | Storage : Store at -20°C long term. Once rehydrated it is recommended to prepare appropriate aliquots and store them at -20°C. Avoid repeated freeze/thaw cycles. | Reconstitution : Prepare sterile stock solution by dissolving rhsFasL in 100 μL sterile water (0.1 mg/mL in PBS). Further dilutions should be made with medium containing 5% fetal calf serum. | Warning : No warranties, expressed or implied, are made regarding the use of this product. Creative Biomart is not liable for any damage, personal injury, or economic loss caused by this product.