Recombinant Human FCER2 protein

Specification :
| Description : The protein encoded by this gene is a B-cell specific antigen, and a low-affinity receptor for IgE. It has essential roles in B cell growth and differentiation, and the regulation of IgE production. This protein also exists as a soluble secreted form, then functioning as a potent mitogenic growth factor. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. | Source : E.coli | Species : Human | Form : Lyophilized from a 0.2μm filtered concentrated solution in PBS, pH 7.4. | Bio-activity : Fully biologically active when compared to standard. The ED50 determined by its ability to induce TNF-alpha production by human PBMCs. | Molecular Mass : Approximately 19.2 kDa, a single non-glycosylated polypeptide chain containing 172 amino acids. | Protein length : 172 | AA Sequence : MELQVSSGFVCNTCPEKWINFQRKC YYFGKGTKQWVHARYACDDMEGQLV SIHSPEEQDFLTKHASHTGSWIGLR NLDLKGEFIWVDGSHVDYSNWAPGE PTSRSQGEDCVMMRGSGRWNDAFCD RKLGAWVCDRLATCTPPASEGSAES MGPDSRPDPDGRLPTPSAPLHS | Endotoxin : Less than 0.1 EU/µg of rHusCD23 as determined by LAL method. | Purity : >95% by SDS-PAGE and HPLC analysis. | Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. | Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.