Active Recombinant Human FCGR1A(Gln 16 – Pro 288), His-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His | Protein length : Gln 16 – Pro 288 | Conjugation/Label : Biotin | Form : Lyophilized from 0.22 μm filtered solution in PBS, pH7.4, 10% Trehalose. | Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized Yervoy Ipilimumab (Human IgG1) at 10 μg/mL (100 μL/well) can bind Biotinylated Human Fc gamma RI with a linear range of 1.5-50 ng/mL. | Molecular Mass : This protein carries a 15 AA fragment at the C-terminus, followed by a polyhistidine tag. There is one single lysine residue within the 15 AA fragment that is enzymatically labeled with biotin in vivo. The protein has a calculated MW of 34.3 kDa. The predicted N-terminus is Gln 16. The reducing (R) protein migrates as 45-60 kDa in SDS-PAGE due to glycosylation. | Endotoxin : | Purity : >92% as determined by SDS-PAGE. | Storage : Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.