Active Recombinant Human FCGR2A protein, hFc-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : hFc | Form : Lyophilized from sterile PBS, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. | Bio-activity : Measured by its binding ability in a functional ELISA.Immobilized human CD32a-Fc at 10 μg/ml (100 μl/well) can bind biotinylated human IgG1 with a linear range of 0.625-10 μg/ml. | Molecular Mass : The recombinant human CD32a/Fc is a disulfide-linked homodimeric protein. The reduced monomer consists of 426 amino acids and predicts a molecular mass of 47.3 kDa. As a result of glycosylation, the apparent molecular mass of rh CD32a/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions. | Protein Length : Met1-Ile218, 167Arg | Endotoxin : | Purity : > 90 % as determined by SDS-PAGE | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°CStore it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.