Active GMP Recombinant Human HGF Protein

Specification :
| Source : Mammalian cells | Species : Human | Form : Lyophilized from a 0.2 μm filtered solution in PBS. | Bio-activity : Measured by its ability to induce IL-11 secretion by Saos‑2 human osteosarcoma cells. Hjertner, O. et al. (1999) Blood 94:3883.The specific activity of recombinant human HGF is approximately 1.5 x 10^3 IU/μg, which is calibrated against recombinant human HGF WHO International Standard (NIBSC code: 96/564). | Molecular Mass : Predicted Molecular Mass: alpha chain: 53.7; beta chain: 26 kDaSDS-PAGE: 65-75 kDa, non-reducing conditions 60-65 kDa, and 30-40 kDa, reducing conditions | N-terminal Sequence Analysis : Amino acid sequencing of the alpha chain was blocked, suggesting it is consistent with Gln32Gln32-Arg-Lys-Arg-Arg-Asn-Thr-Ile-His-Glu (alpha -chain)Val495-Val-Asn-Gly-Ile-Pro-Thr-Arg-Thr-Asn (beta -chain) | Endotoxin : | Purity : >95%, by SDS-PAGE with silver staining, under reducing conditions. | Storage : Avoid repeated freeze-thaw cycles.12 months, -20 to -70 centigrade as supplied.1 month, 2 to 8 centigrade under sterile conditions after reconstitution.3 months, -20 to -70 centigrade under sterile conditions after reconstitution. | Reconstitution : Reconstitute at 100 μg/mL in PBS. | Quality Statement : Our GMP proteins are produced according to relevant sections of the following documents: WHO TRS, No. 822, 1992 Annex 1, Good Manufacturing Practices for Biological Products; USP Chapter 1043, Ancillary Materials for Cell, Gene and Tissue-Engineered Products and USP Chapter 92, Growth Factors and Cytokines Used in Cell Therapy Manufacturing. | Shipping : The product is shipped at ambient temperature. Upon receipt, store it immediately at the recommended temperature.