Active Recombinant Human FCGR2A protein, His-tagged

Specification :
| Source : HEK293 | Species : Human | Tag : His | Form : Lyophilized from sterile PBS, pH 7.5Please contact us for any concerns or special requirements. Normally 5 % – 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization. Please refer to the specific buffer information in the hard copy of CoA. | Bio-activity : 1. Measured by its ability to bind biotinylated human IgG2 in functional ELISA.2.Loaded Rituximab (IgG1) on ProA Biosensor, can bind Human FcγRIIA / CD32a (R167) recombinant protein with an affinity constant of 2.3 μM as determined in a BLI assay (ForteBio Octet Red384).3.Loaded Human FcγRIIA / CD32a (R167) recombinant protein on His1K Biosensor, can bind Bevacizumab (IgG1) with an affinity constant of 0.65 μM as determined in a BLI assay (ForteBio Octet Red384).4.Captured Bevacizumab (IgG1) on proA Chip can bind Human FcγRIIA / CD32a (R167) recombinant protein with an affinity constant of 0.79 µM as determined in an SPR assay (Biacore T200). | Molecular Mass : The recombinant human FCGR2A consists of 196 amino acids after removal of the signal peptide and predicts a molecular mass of 22 kDa. It migrates as an approximately 30 kDa protein due to glycosylation by SDS-PAGE under reducing conditions. | Proteinlength : Met1-Ile218,167Arg | Endotoxin : | Purity : > 95 % as determined by SDS-PAGE. > 90 % as determined by SEC-HPLC | Storage : Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents. | Publication : The Characterisation of FcγRIIa Antagonists (2016)