Active Recombinant Human FCGRT/B2M Protein, His & Strep & Avi-tagged, Biotinylated

Specification :
| Source : HEK293 | Species : Human | Tag : His & Strep & Avi | Predicted N Terminal : Ala 24(FcRn) & Ile 21(B2M) | Form : Lyophilized from sterile PBS, pH7.4, 10% Trehalose. | Bio-activity : Measured by its binding ability in a functional ELISA against immobilized Yervoy Ipilimumab (Human IgG1, 1μg/well). The linear range is 0.3-5 μg/mL. Biotinylated Human FcRn / FCGRT & B2M, Avi Tag coupled to SA coated sensor chip can bind Herceptin with an affinity constant of 2.2-2.6 μM as determined in a SPR assay (Biacore 2000). | Molecular Mass : The subunit FCGRT / FcRn carries an Avi tag at the C-terminus, followed by a polyhistidine tag. The subunit Beta-2 microglobulin (B2M) carries a Strep II tag at the C-terminus. The protein has a calculated MW of 33 kDa (FcRn), 13.1 kDa (B2M). The protein migrates as 36 kDa (FCGRT), 14 kDa (B2M) respectively on a SDS-PAGE gel under reducing condition due to glycosylation. | Endotoxin : Less than 1.0 EU per μg by the LAL method. | Purity : >95% as determined by SDS-PAGE. | Stability : Samples are stable for up to twelve months from date of receipt at -70ºC. | Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles. | Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4℃ before opening to recover the entire contents.