Recombinant Human Fibroblast Growth Factor

Specification :
| Cat. No. : FGFR2-102H | Description : This protein functions as a modifier of endothelial cell migration and proliferation, as well as an angiogenic factor. It acts as a mitogen for a variety of mesoderm- and neuroectoderm-derived cells in vitro, thus is thought to be involved in organogenesis. Three alternatively spliced variants encoding different isoforms have been described. The heparin-binding growth factors are angiogenic agents in vivo and are potent mitogens for a variety of cell types in vitro. There are differences in the tissue distribution and concentration of these 2 growth factors. | Source : Baculovirus. | Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Ala-Ala-Gly-Ser-Ile. | Physical Appearance : Sterile Filtered liquid formulation. | Purity : Greater than 98.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. | Formulation : The sterile protein solution (0.5 mg/ml) contains 20 mM Tris pH=7.9, 100 mM KCl, 1 mM DTT and 20% glycerol. | Specific Activity : The ED50, calculated by the dose-dependant proliferation of BAF3 cells expressing FGF receptors (measured by 3H-thymidine uptake) is | Protein Content : Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.8511 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of Fibroblast Growth Factor-b as a Reference Standard. | Stability : Fibroblast Growth Factor-basic although stable at 4°Cfor 3 weeks, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.