Recombinant Human FZD8 protein, His-Avi-tagged, Biotinylated

Specification :
| Description : Frizzled-8 is one of at least ten seven-transmembrane (7TM) glycoproteins of the Frizzled family of Wnt receptors. Frizzled proteins are thought to be G-protein-coupled. Wnt engagement, with low density lipoprotein receptor-related proteins LRP-5 or LRP-6 acting as co-receptors, stabilizes beta -catenin and promotes gene transcription that is important in development and tissue maintenance. Component of the Wnt-Fzd-LRP5-LRP6 complex that triggers beta-catenin signaling through inducing aggregation of receptor-ligand complexes into ribosome-sized signalosomes. The beta-catenin canonical signaling pathway leads to the activation of disheveled proteins, inhibition of GSK-3 kinase, nuclear accumulation of beta-catenin and activation of Wnt target genes. These ligands bind the extracellular CRD of Frizzled-8, blocking Wnt binding. The recombinant Frizzled-8 CRD has also been used to block Wnt signaling and inhibit growth of teratocarcinomas. | Source : HEK293 | Species : Human | Tag : His-Avi | Form : Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. | Molecular Mass : 25-35kDa, reducing conditions. | Protein length : Ala28-Pro172 | Endotoxin : Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test. | Purity : Greater than 95% as determined by reducing SDS-PAGE. | Storage : Lyophilized protein should be stored at Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at | Reconstitution : Always centrifuge tubes before opening. Do not mix by vortex or pipetting.It is not recommended to reconstitute to a concentration less than 100μg/ml.Dissolve the lyophilized protein in distilled water.Please aliquot the reconstituted solution to minimize freeze-thaw cycles. | Shipping : The product is shipped at ambient temperature.Upon receipt, store it immediately at the temperature listed below.