When cultured below substantial-zinc situations, substantial luciferase exercise was noticed in ZnT12/2MT2/2ZnT42/two cells and the cells expressing the W152R hZnT2 mutant, but the activity was markedly decreased in the cells expressing the WT hZnT2 or S296L hZnT2 mutant
For immunoprecipitation, the membrane factions geared up from cells lysed in NP-forty buffer [twenty...